Video observations on human blastocyst hatching
نویسندگان
چکیده
منابع مشابه
Time-lapse videomicrographic observations of blastocyst hatching in cattle.
Morphological changes of cultured bovine blastocysts during hatching were observed using time-lapse videomicrography in order to investigate the patterns of the hatching process that occurred in the blastocysts and to determine whether the hatching patterns differed between blastocysts developed from fresh and cryopreserved embryos. Compacted morulae (CMs) were collected from superovulation-tre...
متن کاملUltrastructural observations of enzymatically treated human blastocysts: zona-free blastocyst transfer and rescue of blastocysts with hatching difficulties.
Enzymatic treatment of the zona pellucida to either soften or remove totally the zona before blastocyst transfer has resulted in high implantation rates. The zona is usually completely dissolved after 1.5 min exposure with 10 IU pronase at 37 degrees C. Since there may be concerns that pronase treatment for periods of 1.5 min or longer may cause adverse effects on the trophectoderm (TE) and inn...
متن کاملLiver receptor homolog 1 influences blastocyst hatching in pigs
Liver receptor homolog 1 (Lrh1, also known as Nr5a2) belongs to the orphan nuclear receptor superfamily and has diverse functions in development, metabolism, and cell differentiation and death. Lrh1 regulates the expression of Oct4, which is a key factor of early embryonic differentiation. However, the role of Lrh1 in early development of mammalian embryo is unknown. In the present study, the l...
متن کاملRelevance of Assisted Hatching with Blastocyst Stage Transfer
When a blastocyst expands with the growth of the blastocoel, it stretches and thins the zona pellucida (ZP), ultimately rupturing the ZP allowing the embryo to hatch free ready for implantation. In the event this does not occur, then an otherwise potentially viable embryo may not implant. Assisted hatching (AH) has been developed to overcome this, but has been applied mostly to day-2/3 embryos ...
متن کاملTranscriptome analysis in blastocyst hatching by cDNA microarray.
BACKGROUND Hatching is an important process for early embryo development, differentiation and implantation. However, little is known about its regulatory mechanisms. By integrating the technologies of RNA amplification and cDNA microarrays, it has become possible to study the gene expression profile at this critical stage. METHODS Pre-hatched and hatched ICR mouse embryos (25 blastocysts in e...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2003
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61755-5